Table 3.

Primer sets designed in this study and corresponding templates.

Template	Oligonucleotide	Sequence (5′ to 3′)	Annealing temperature (°C)
pLAEG2_dhps01	AEG2_dhps01_for_150	GATACCCTAACGTACTACCGC	55
	AEG2_dhps01_rev	TCAGCGCGGATTCGTTC	55
pLAEW9_dhps01	AEW9_dhps01_for_150	CCTGATCGGTCAGGTCCTTA	55
	AEW9_dhps01_rev	TTACGCCGTTTGGCCC	55
pLSEW2_dhps01	SEW2_dhps01_for_150	CCGCCCGCCGTGTG	60
	SEW2_dhps01_rev	TTATGAAGCGGCGATAGCAGTAATAAC	60
pLSEW5_dhps01	SEW5_dhps01_for_104	GGTCATCGCGACAAAGGGTG	60
	SEW5_dhps01_rev	CTATACAGGCCGTCCAGCTGC	60
pLAEG3_tet01	AEG3_tet01b_for	CTATTGCTTGACGCGATCG	55
	AEG3_tet01a_rev	CTATTCCGCCGGCTCAG	55
pLSEG6_tet01	SEG6_tet01b_for	TTATCCTCGACGCGCCTTG	60
	SEG6_tet01a_rev	TCAGCCCGGAGCCAAGG	60
pLSEG8_tet01	SEG8_tet01_for_150	GGATTTGGAACAGACATATAGTG	55
	SEG8_tet01_rev	TTACCGGTTCCCCACTG	55
pLSEG8_tet02	SEG8_tet02_for_150	TTTAAGAGAATTTTCAGGATAAAG	50
	SEG8_tet02_rev	TTAACCATGCTTTGTCAG	50