Table 4.
Characterization of soil metagenomic libraries and designation of plasmids harbored by positive clones.
| Library | Number of clones | Average insert size (kb) | Insert frequency (%) | Estimated library size (Gb) | Plasmids of positive clones |
|---|---|---|---|---|---|
| AEG2 | 115965 | 3.6 | 73 | 0.30 | pLAEG2_dhps01 |
| AEG3 | 40095 | 5.8 | 85 | 0.20 | pLAEG3_tet01 |
| AEW9∗ | 100950 | 2.6 | 89 | 0.23 | pLAEW9_dhps01 |
| SEG6∗ | 39825 | 6.0 | 91 | 0.22 | pLSEG6_tet01 |
| SEG8 | 559000 | 4.8 | 86 | 2.30 | pLSEG8_tet01-02 |
| SEW2∗ | 135240 | 5.7 | 95 | 0.73 | pLSEW2_dhps01 |
| SEW5∗ | 166040 | 4.0 | 95 | 0.63 | pLSEW5_dhps01 |
AEG/AEW: metagenomic libraries derived from the Biodiversity Exploratory Schwäbische Alb; SEG/SEW: metagenomic libraries derived from the Biodiversity Exploratory Schorfheide-Chorin. ∗Previously generated libraries. Names of constructed metagenomic libraries refer to the designation of the samples from which the libraries were derived (see Table 2).