Table 6.
Antibiotic susceptibility of plasmid-carrying E. coli clones.
| Plasmid | Minimal inhibitory concentration (μg/ml) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAX | CHL | ERY | GEN | LIN | RIF | SDZ | SMX | SMZ | SOX | TET | TYL | |
| Cloning vector | <0.125 | 2 | 1024 | 4 | 512 | 8 | 15.625 | 7.8125 | 62.5 | 15.625 | 1 | 512 |
| pCR4_AEG2_dhps01 | <0.125 | 2 | 1024 | 4 | 512 | 8 | 62.5 | 31.25 | 500 | 62.5 | 1 | 512 |
| pCR4_AEW9_dhps01 | <0.125 | 2 | 1024 | 4 | 512 | 8 | 125 | 500 | 250 | 1000 | 1 | 512 |
| pCR4_SEW2_dhps01 | <0.125 | 2 | 1024 | 4 | 512 | 8 | 500 | 500 | >1000 | 1000 | 1 | 512 |
| pCR4_SEW5_dhps01 | <0.125 | 2 | 1024 | 4 | 512 | 8 | >1000 | 500 | >1000 | 1000 | 1 | 512 |
| pCR4_AEG3_tet01ab | <0.125 | 2 | 1024 | 4 | 2048 | 8 | 15.625 | 7.8125 | 62.5 | 15.625 | 2 | 512 |
| pCR4_SEG6_tet01ab | <0.125 | 2 | 1024 | 4 | 512 | 8 | 15.625 | 7.8125 | 62.5 | 15.625 | 2 | 512 |
| pCR4_SEG8_tet01 | <0.125 | 2 | 1024 | 4 | 512 | 8 | 15.625 | 7.8125 | 62.5 | 15.625 | 8 | 512 |
| pCR4_SEG8_tet02 | <0.125 | 2 | 1024 | 4 | 512 | 8 | 15.625 | 7.8125 | 62.5 | 15.625 | 2 | 512 |
CAX, cefotaxime; CHL, chloramphenicol; ERY, erythromycin; GEN, gentamicin; LIN, lincomycin; RIF, rifampicin; SDZ, sulfadiazine; SMX, sulfamethoxazole; SMZ, sulfamethazine; SOX, sulfisoxazole; TET, tetracycline; TYL, tylosin. Bold values indicate an increase in minimal inhibitory concentration compared to the control strain carrying the cloning vector pCR4-TOPO.