Fig. 4.

In vivo performance of NP_PPBBT nanoparticles on tumours. a Blood DiD concentration vs. time curves in healthy mice or orthotopic EMT-6 breast tumour-bearing mice intravenously injected with NP_PPBBT/DiD at a DiD dose of 0.25 mg kg⁻¹ (n = 3 for each group). b Quantification of DiD fluorescence from the major organs (heart, liver, spleen, lung, kidney, and tumours) in orthotopic EMT-6 tumour-bearing mice sacrificed at 12, 24, 48 or 72 h post injection of NP_PPBBT/DiD at a DiD concentration of 0.25 mg kg⁻¹. c, d IR thermal images (the tumours were indicated by yellow dashed circles) (c) and tumour temperature evolutions (d) of orthotopic EMT-6 tumour-bearing mice after intravenous injection of PBS or 5 mg kg⁻¹ NP_PPBBT under 808 nm laser irradiation at 0.5 or 1.0 W cm⁻² for 5 min. NP-IR: NP_PPBBT plus laser irradiation; PBS-IR: PBS plus laser irradiation. Results are presented as mean ± S.D., n = 3