Fig. 5.
PTT efficiency of NPPPBBT nanopartiles on tumours. a, b Body weight (a) and tumour volume (b) curves of orthotopic EMT-6 tumour-bearing mice at different time points after receiving one dose of treatment with indicated formulations. At 24 h post injection, tumours were irradiated with (or w/o) laser for 10 min and the observations started. c, d Photographs (c) and weights (d) of tumours in mice sacrificed after 15-day observation. e H&E staining, Ki67 (Cy3, red) and DAPI (blue) counterstaining, and TUNEL (FITC, green) and DAPI (blue) counterstaining of the tumour tissues from mice sacrificed at day 15 post treatment. Scale bars, 50 μm for H&E staining; 20 μm for Ki67 and TUNEL staining. PBS-IR: PBS plus laser irradiation; NP-IR: NPPPBBT plus laser irradiation; NP-NO IR: NPPPBBT without laser irradiation. Results are presented as mean ± S.D., n = 5; n.s. not significant, p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, analyzed by Student’s t-test