Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 13;9:4327. doi: 10.1038/s41598-019-40867-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Loss of Rnf2 is directly associated with upregulation of gene expression. (a) MA-plot of differentially expressed genes between wildtypes and rnf2 mutants at 3 dpf. Significantly differentially expressed genes |Log2FC ≥ 1|; padj < 0.1 are highlighted in red. In total 292 genes are upregulated and 200 genes are downregulated. (b) Gene Set Enrichment Analysis (GSEA) for the genes whose promoters belong to the five clusters defined in Fig. 2a. Cluster 1, 2, 4, and 5 show a non-significant distribution of the genes based on their expression changes upon the rnf2 mutation. Genes belonging to the promoters from cluster 3 are enriched for upregulation upon mutation of rnf2. p-value = < 0.001; NES = 2.04. (c) Gene ontology of biological processes analysis of the 112 genes belonging to cluster 3. (d) ChIP-seq and RNA-seq coverage at the lbx1a gene and the tal1 gene in 3 dpf zebrafish embryos. Light blue: Rnf2 ChIP-seq tracks in wildtypes. Teal: Rnf2 ChIP-seq tracks in rnf2 mutants. Orange: H3K27me3 ChIP-seq track in wildtypes. Brown: H3K27me3 ChIP-seq track in rnf2 mutants. Lilac: wildtype input. Green: RNA-seq track in wildtypes. Red: RNA-seq track in rnf2 mutants.