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. 2019 Mar 13;9:4360. doi: 10.1038/s41598-019-39538-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Azathioprine negatively modulates the intrinsic adipogenic potential of FAPs purified from wild type and mdx mice. (A) Schematic representation of the procedure used to purify muscle precursor cells from the hind limbs of wild type and mdx mice. (B) Schematic representation of the differentiation protocol applied to induce the adipogenic differentiation of wild type and mdx FAPs in vitro. (C) Representative immunofluorescence (20x magnification) of FAP-derived adipocytes from wild type and mdx mice differentiated in the presence of increasing concentrations (1, 10, 25, 50 μM) of AZA or vehicle alone. Adipocytes (red) were stained with ORO solution and nuclei (blue) with Hoechst 33342. (D) The floating bar graph represents the average area (expressed in pixel) of ORO staining per cell in each field for each experimental condition. (E) Floating bar graph represents the average of the percentage ratio between ORO-positive cells and the number of cells in each field for each experimental condition. (F) Floating bar graph represents the average number of nuclei per field in each experimental condition. All experimental data are represented as means of three independent experiments ± SEM. The statistical significance was estimated by one way ANOVA and defined as *p < 0.05; **p < 0.01; ***p < 0.001. Scale bar: (C) 100 μm.