Fig. 2. Lgals3 loss-of-function alleles used in this study.

Schematic diagrams showing the genetic perturbations to the wild-type allele including (a) the Lgals3-KO allele, which was generated by replacing exons 2–4 with a Neo selection cassette and (b) the allele generated by EUCOMM that inserted a lacZ reporter and Neo selection cassette flanked by FRT sites and an exon 4 flanked by loxP sites. The lacZ and Neo cassettes were removed following a cross to a mouse expressing the FLP recombinase transgene thereby generating the Lgals3-flox allele. Mice with the Lgals3-flox allele were then crossed with a mouse expressing the CMV-Cre transgene to obtain mice with a new genomic Lgals3-null allele (Lgals3-∆).