Fig. 5. Increased osteoblastogenesis and normal osteoclastogenesis observed following differentiation of Lgals3-deficient bone cells in vitro.

Calvarial osteoblasts from 2 animals per genotype were cultured for 6 d in osteogenic conditions. a Differentiation was analyzed by alkaline phosphatase (ALP) staining (Abs = 405 nm) relative to DNA content (Hoechst 33342; 350 nm, 461 nm) and normalized to wild-type ratios. In identical cultures, gene expression was assessed by qPCR (b–e). The results represent the mean ± S.E.M. and are representative of duplicate experiments. Bone marrow myeloid cells from 1 animal per sex per genotype were differentiated into osteoclasts for 5 d in osteoclastic conditions. f Representative images of osteoclast cultures. Osteoclast counts for male (g) and female (h) osteoclast differentiation experiments.