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. 2019 Mar 13;10:1193. doi: 10.1038/s41467-019-09114-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Selectively knocking out Efr3a in the CA3 area prevents oligomeric Aβ-induced inhibition of presynaptic release probability at the SC-CA1 synapse. a–j Representative traces of PPF (b, CA1-Efr3a cKO mice; g, CA3-Efr3a cKO mice) and 20 Hz train responses (d, CA1-Efr3a cKO mice; i, CA3-Efr3a cKO mice) and quantification of PPF ratio (c, CA1-Efr3a cKO mice; h, CA3-Efr3a cKO mice), RRP size (e, CA1-Efr3a cKO mice; j, CA3-Efr3a cKO mice; left), and Pr (e, CA1-Efr3a cKO mice; j, CA3-Efr3a cKO mice; right) at the SC-CA1 synapse showing oligomeric Aβ (400 nM) increases Pr in CA1-Efr3a cKO mice (immunoblotting and quantification of Efr3a in CA1 shown in a), whereas oligomeric Aβ no longer changes Pr in CA3-Efr3a cKO mice (immunoblotting and quantification of Efr3a in CA3 shown in f). t test; *P < 0.05; **P < 0.01; ***P < 0.001; N = 3–7 per group. k–r Representative traces of PPF (k, APP/PS1 CA1-Efr3a cKO and control mice; o, APP/PS1 CA3-Efr3a cKO and control mice) and 20 Hz train responses (m, APP/PS1 CA1-Efr3a cKO and control mice; q, APP/PS1 CA3-Efr3a cKO and control mice) and quantification of PPF ratio (l, APP/PS1 CA1-Efr3a cKO and control mice; p, APP/PS1 CA3-Efr3a cKO and mice), RRP size (n, APP/PS1 CA1-Efr3a cKO and control mice; r, APP/PS1 CA3-Efr3a cKO and control mice; left), and Pr (n, APP/PS1 CA1-Efr3a cKO and control mice; r, APP/PS1 CA3-Efr3a cKO and mice; right) at the SC-CA1 synapse showing selectively knocking out Efr3a in the CA3 area restores the decreased Pr in APP/PS1 mice. One-way ANOVA with post hoc Dunnett’s test; F_(2,29) = 11.38 (l); F_(2,31) = 20.66 (p); F_(2,26) = 0.28 (n, left); F_(2,26) = 5.99 (n, right); F_(2,21) = 0.986 (r, left); F_(2,21) = 0.19 (r, right); *P < 0.05; **P < 0.01; ***P < 0.001; N = 8–15 per group. Data are mean ± SEM. Source data are provided as a Source Data file