Fig. 3.

SOS2^Ser294 mutation rescues the salt-sensitive phenotype of pks5-4. a Salt sensitive analysis of BigM, pks5-3, and pks5-4. Five-day-old seedlings were transferred from MS to medium containing 75 or 100 mM NaCl. Photographs were taken 10 days after transfer. b Root length analysis of seedlings in (a). Error bars represent SD; p ≤ 0.05, Student’s t-test; n = 12. c Net Na⁺ flux analysis at the meristem zone of BigM, pks5-3, and pks5-4 by NMT. Five-day-old seedlings were pretreated with 100 mM NaCl for 24 h. Error bars represent SD; p ≤ 0.05, Student’s t-test; n = 6. d Calculated net Na⁺ fluxes from (c). e Salt sensitive analysis of pks5-4 and transgenic plants expressing Myc-SOS2 or Myc-SOS2^S294A in the pks5-4 background. S2, Pro35S:6×Myc-SOS2 in pks5-4; S2^SA, Pro35S:6×Myc-SOS2S294A in pks5-4. Photographs were taken 10 days after transfer. f Root length analysis of seedlings in (e). Error bars represent SD; p ≤ 0.05, Student’s t-test; n = 12. g Net Na⁺ flux analysis at the meristem zone of pks5-4 and different transgenic plants by NMT. Error bars represent SD; p ≤ 0.05, Student’s t-test; n = 6. h Calculated net Na⁺ fluxes from (g). For b, d, f, and h, significant differences are indicated by different lowercase letters. Source data of b–d and f–h are provided in the Source Data file