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. 2019 Mar 13;10:1199. doi: 10.1038/s41467-019-09181-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — 14-3-3 proteins repress the kinase activity of PKS5. a In vitro kinase assay showing that 14-3-3λ and κ proteins inhibit PKS5 activity in a dose-dependent manner. Recombinant His-tagged PKS5 was incubated with GST-tagged SCaBP1 with or without GST-tagged 14-3-3 proteins. SCaBP1 was used as the substrate and 14-3-3ω served as a negative control. b, c Kinase assays of PKS5 in Col-0 and 14-3-3λκ double mutant backgrounds under salt stress. Myc-PKS5 was purified from Pro35S:6×Myc-PKS5 transgenic plants treated with 100 mM NaCl for the indicated periods. MBP was used as the substrate. CBB Coomassie Brilliant Blue, Autorad autoradiograph, IB immunoblot. Source data are provided as a Source Data file