Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 13;10:1199. doi: 10.1038/s41467-019-09181-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — Overexpression of 14-3-3 rescues the phenotypes of pks5-4. a Alkaline sensitive analysis of BigM, pks5-4, and two independent transgenic lines (OE-3 and OE-4) expressing Flag-HA-14-3-3λ driven by the 35S promoter in the pks5-4 background. Seven-day-old seedlings were transferred to pH 6.5 or 6.7 MS medium adjusted using NaHCO₃. Photographs were taken 10 days after seedling transfer. b Comparison of PM H⁺-ATPase activity in BigM, pks5-4, OE-3, and OE-4. Plasma membrane vesicles were purified from 5-week-old seedlings grown in soil treated with 250 mM NaCl for 3 days. Error bars represent SD; p ≤ 0.05, Student’s t-test; n = 3; significant differences are indicated by different lowercase letters. c Timely varying curves of PM H⁺-ATPase activity in BigM, pks5-4, OE-3, and OE-4. d Alkaline tolerance analysis of Col-0, pks5-1, and two independent transgenic lines (OE-1 and OE-2) expressing Flag-HA-14-3-3λ driven by the 35S promoter in the pks5-1 background. Photographs were taken 10 days after seedling transfer. e Comparison of PM H⁺-ATPase activity in Col-0, pks5-1, OE-1, and OE-2. Error bars represent SD; p ≤ 0.05, Student’s t-test; n = 3; significant differences are indicated by different lowercase letters. f Timely varying curves of PM H⁺-ATPase activity in Col-0, pks5-1, OE-1, and OE-2. Source data of b, c, e, and f are provided in the Source Data file