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. 2019 Mar 13;9:4337. doi: 10.1038/s41598-019-40663-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hematopoietic deletion of Dectin-2 or CARD9 hardly affects immune cell composition in the circulation or in bone marrow. At the end of the study, after 10 weeks of Western-type diet feeding, immune cells subsets in the blood of Ldlr^−/− mice transplanted with BM from control (WT), Dectin-2^−/− or Card9^−/− were determined by flow cytometry. The amount of innate immune cell subsets are shown for (A) total Ly6C monocytes, which were subdivided into (B) Ly6C^hi-, (C) Ly6C^med-, (D) Ly6C^lo-monocytes. Further, the percentage of (E) neutrophils, (F) eosinophils and (G) dendritic cells is shown. The percentage of adaptive immune cell subsets is determined for (H) B-cells, (I) total T-cells and T-cell subsets such as (J) cytotoxic T-cells and (K) T-helper cells. Data are presented as mean ± SEM. n = 7–8/group.