Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 13;9:4373. doi: 10.1038/s41598-019-40760-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

HMBA downmodulates NKG2D on NK cells. (A) The purity of NK cells isolated from PBMCs was examined by flow cytometry measuring the frequency of CD3⁻CD56⁺CD16^+/− cells. (B,C) NK cells were cultured for 18 h in medium alone (nt) or supplemented with 5 mM HMBA and examined for the expression of various markers: (B) the percentage of DEAD⁺, CD69⁺ and CD107a⁺ NK cells among nt (filled grey histograms, grey percentages) and treated cells (open histograms, black percentages) is shown together with control IgG signal (dashed line) for a representative experiment; (C) both the % of positive cells and MFI for NKG2D, DNAM-1, NKp46, NKp44, NKp30, and CD16 are shown for NK cells nt and treated with HMBA and/or 12.5 ng/ml of IL-15. Experiments were performed with at least 5 independent donors. Bars represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by two-tailed paired t test.