Table 2.
KD (nm) for interactions between PAI-1 and uPA variants
| uPA | Mammalian P3 |
Non-mammalian P3 |
||
|---|---|---|---|---|
| hPAI-1 WT | zfPAI-1 Y(P3)S | hPAI-1 S(P3)Y | zfPAI-1 WT | |
| huPA S195A | 0.58 ± 0.04 (3) | 1.04 ± 0.08 (3) | 17.1 ± 0.69 (3) | 5.39 ± 0.51 (3) |
| huPA L97bG/S195A | 22.8 ± 2.98 (3) | 15.7 ± 0.58 (3) | 8.83 ± 1.28 (3) | 8.29 ± 0.36 (3) |
| huPA S195A/R217E | 0.06 ± 0.01 (3) | 1.47 ± 0.37 (3) | 1.52 ± 0.16 (3) | 2.03 ± 0.15 (3) |
| huPA L97bG/S195A/R217E | 2.85 ± 0.78 (3) | 21.4 ± 5.57 (3) | 0.46 ± 0.15 (3) | 0.35 ± 0.12 (3) |
| huPA L97bG/H99Y/S195A/R217E | 26.1 (1) | 30.93 (1) | 2.96 (1) | 20.98 (1) |
| zfuPA S195A | 2.21 ± 0.24 (3) | 5.96 ± 1.25 (3) | 0.05 ± 0.01 (3) | 0.11 ± 0.01 (3) |
| zfuPA G97bL/S195A | 2.70 ± 0.43 (3) | 6.76 ± 1.79 (2) | 0.05 ± 0.01 (3) | 0.17 ± 0.01 (3) |
| zfuPA S195A/E217R | 18.6 ± 5.14 (3) | 34.2 ± 5.54 (3) | 1.32 ± 0.15 (3) | 1.27 ± 0.08 (3) |
| zfuPA G97bL/S195A/E217R | 17.0 ± 3.12 (3) | 28.5 ± 4.35 (3) | 1.04 ± 0.22 (3) | 1.40 ± 0.21 (3) |
| zfuPA G97bL Y99H S195A E217R | 21.89 (1) | 37.06 (1) | 5.66 (1) | 5.49 (1) |