Table 2.
Affinities for binding of C-C motif chemokines to CCR1
Binding was determined using a radioligand (125I-CCL3) displacement assay with membranes prepared from cells grown in the absence of chlorate or the presence of 30 mm chlorate to inhibit sulfation for 48 h prior to membrane preparation. CCR1 expression was induced by the addition of 10 μg/ml tetracycline to the cell medium 24 h prior to membrane preparation. Inhibition constants are reported as pIC50 values (−log10 of the IC50; in m) ± S.E. The corresponding IC50 values (in nm) are shown in parentheses. CCL7 was used as a reference for statistical analysis. *, p < 0.05; ***, p < 0.001.
| Chemokine | pIC50 (no chlorate treatment) | pIC50 (chlorate-treated cells) |
|---|---|---|
| m | m | |
| CCL2 | 7.2 ± 0.1 (57.5)*** | 7.7 ± 0.1 (19.1)*** |
| CCL5 | 9.2 ± 0.2 (0.6) | 9.7 ± 0.1 (0.2) |
| CCL7 | 9.6 ± 0.1 (0.4) | 9.8 ± 0.1(0.2) |
| CCL8 | 8.7 ± 0.2 (1.8)* | 8.7 ± 0.1 (1.9)*** |
| CCL15 | 10.1 ± 0.2 (0.07) | 10.0 ± 0.1 (0.1) |
| CCL26 | No binding | 6.3 ± 0.2 (457.1)*** |