Figure 4.
LysM-resistance mutations map to TMD2 and TMD7 of MurJ. The amino acid changes in E. coli MurJ (MurJEC) resulting in LysM resistance were mapped onto the structure of MurJ from Thermosipho africanus (MurJTA) (PDB code 5T77). A, cytoplasmic-open conformation. B, model of the periplasmic-open conformation (19). The TMDs that line the central hydrophilic cavity are colored: TMD2 (light blue) and TMD7 (magenta). Left, lateral view; right, periplasmic view. The changes in MurJEC and homologous amino acids in MurJTA are shown on the right. This research was originally published in Nature Microbiology. Chamakura, K. R., Sham, L. T., Davis, R. M., Min, L., Cho, H., Ruiz, N., Bernhardt, T. G., and Young, R. A viral protein antibiotic inhibits lipid II flippase activity. Nat. Microbiol. 2017; 2:1480–1484. © Nature Research.