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SsaG is required for SsaI secretion. Salmonella strains CS10157 (ΔG/pUHE), CS10155 (ΔG/pG), CS10156 (ΔG/pI), CS10068 (ΔI/pUHE), CS10159 (ΔI/pG), and CS10069 (ΔI/pI) were grown at 37 °C for 8 h in low-Mg2+ minimal medium with 0.1 mm IPTG for the induction of ssaI. Each protein was detected in cell lysates (A) and supernatants (B) by immunoblotting using anti-SseB and anti-SsaI antibodies.
