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. 2019 Jan 16;294(10):3783–3793. doi: 10.1074/jbc.RA118.005072

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© 2019 Takaya et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 3. — SsaH is required for SsaI secretion. A, effect of disruption of ssaH on secretion and production of SseB, SsaI, and SsaH. Each protein was detected in cell lysates and supernatants via immunoblotting using anti-SseB, anti-SsaI, and anti-SsaH antibodies. Bacterial cells of strains χ3306 (WT), CS10091 (ΔssaH; ΔH), CS10095 (ΔH/pH), and CS10096 (ΔH/pUHE) were used. B, ribbon representation of SsaH structure predicted by the SWISS-MODEL expert system. C, amino acid alignment of SsaH and class III chaperones PscG, AscG, and YscG.