Figure 5.

SsaE controls the cellular level of SsaH and SseB. A, effect of disruption of ssaE on secretion and production of SseB, SsaI, and SsaH. Each protein was detected in supernatants and cell lysates via immunoblotting using anti-SseB, anti-SsaI, and anti-SsaH antibodies. Bacterial cells of strains χ3306 (WT), CS10106 (ΔssaE; ΔE), CS10150 (ΔE/pE), and CS10151 (ΔE/pMW) were used. B, overproduction of SsaH in ssaE-disrupted cells did not complement the secretion of SsaI. Bacterial strains χ3306 (WT), CS10153 (ΔE/pUHE), and CS10165 (ΔE/pH) were grown at 37 °C for 8 h in low-Mg²⁺ minimal medium with 0.1 mm IPTG for the induction of ssaH. Each protein in supernatants and cell lysates was detected via immunoblotting using anti-SseB, anti-SsaI, and anti-SsaH antibodies.