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. 2019 Jan 2;294(10):3501–3513. doi: 10.1074/jbc.RA118.005940

Figure 6.

Figure 6.

Analysis of SOD1 and microglia in mouse spinal cord. Lumbar spinal cord sections from euthanized mice were analyzed by IHC. A–C, representative images of pAb 16831, a pan-SOD1 antibody, staining for total mouse and human SOD1 in vehicle-treated (A), 0.5 mg/kg (B), and 5.0 mg/kg (C) CLR01-treated mice. D, quantitative analysis of pAb 16831 staining. E–H, representative images of mAb C4F6, which stains specifically apo G93A-SOD1 in vehicle-treated (E), 0.5 mg/kg (F), and 5.0 mg/kg (G) CLR01-treated mice. H, quantitative analysis of mAb C4F6 staining. I–L, representative images of mAb 10C12, which stains specifically early-forming species of misfolded SOD1 in vehicle-treated (I), 0.5 mg/kg (J), and 5.0 mg/kg (K) CLR01-treated mice. L, quantitative analysis of mAb 10C12 staining. M–P, representative images of anti-Iba1 pAb, which stains microglia in vehicle-treated (M), 0.5 mg/kg (N), and 5.0 mg/kg (O) CLR01-treated mice. P, quantitative analysis of anti-Iba1 staining. The scale bars in E–G and I–K denote 100 μm and are applicable also to M–O. **, p < 0.01, one-way ANOVA with post hoc Tukey test.