Figure 2.

Iron depletion and Fe(II) sequestration by CP promote pyoverdine production. A and B, HPLC fluorescence detection (λ_ex = 398 nm, λ_em = 455 nm) for supernatants from PA14 grown in Tris/TSB in the absence or presence of CP-Ser (10 μm) (A) and PA14 grown in Tris/TSB in the absence or presence of CP-Ser, ΔHis₃Asp, ΔHis₄, and ΔΔ (B). Cultures were grown at 37 °C for 8 h. Three biological replicates were performed, and representative chromatograms are shown. Average culture A₆₀₀ values ranged from 1.6 to 2.6 (Table S6).