Figure 11.

Binding of FnBPB to H3 in the presence of PLG. A, recombinant FnBPB(163–480) was immobilized on the surface of microtiter wells. Saturating concentrations of PLG were added along with increasing concentrations of H3. Bound PLG was detected with rabbit anti-PLG IgG followed by HRP-conjugated goat anti-rabbit IgG (solid squares). In the same panel, binding of increasing amounts of H3 to the wells is also reported (open circles). Bound H3 was detected with rabbit anti-histone antibodies followed by HRP-conjugated goat anti-rabbit IgG. B, ELISA-type assay with rFnBPB immobilized on the surface of microtiter wells. Saturating concentrations of H3 were added along with increasing amounts of PLG. Bound H3 was detected with rabbit anti-histones IgG followed by HRP-conjugated goat anti-rabbit IgG (solid squares). The panel shows binding of increasing amounts of PLG to the wells (open circles). Bound proteins were detected with specific antibodies as in A. The data points reported in the panels are the means ±S.D. of three independent experiments each performed in triplicate.