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. 2019 Jan 8;294(10):3588–3602. doi: 10.1074/jbc.RA118.005707

Figure 2.

Figure 2.

Binding of recombinant CWA proteins to immobilized CTH. A, microtiter wells coated with CTH were incubated with purified recombinant A domains of the indicated CWA proteins of S. aureus. Bound proteins were detected with mouse anti-hexahistidine mAb 7E8 followed by HRP-conjugated rabbit anti-mouse IgG. B, microtiter wells coated with CTH were incubated with increasing concentrations of purified recombinant N2N3 domains of FnBPA (FnBPA(194–511)) or FnBPB (FnBPB(163–480)). Bound proteins were detected as described above. The data points are the means (±S.D.) of two independent experiments, each performed in triplicate.