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. 2019 Jan 8;294(10):3588–3602. doi: 10.1074/jbc.RA118.005707

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© 2019 Pietrocola et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 4. — Size-exclusion chromatography analysis of the interaction of FnBPB(163–480) with histone H3. A, elution profiles of FnBPB(163–480), histone H3 alone, and a mixture of a co-incubated, equimolar concentrations of FnBPB and H3 loaded onto a Superdex 75 10/300 GL gel-filtration chromatography column. B, elution profile of a mixture of co-incubated, equimolar concentrations of FnBPB(163–480) trench mutant variant (N312A/F314A) and histone H3 loaded onto the gel-filtration chromatography column as indicated in A. In the inset, a calibration curve relating the K_av of standard proteins to their molecular mass is reported. The figure is representative of three independent experiments. mAU, milli-absorbance units.