Figure 8.

FnBPB expression on the surface of S. aureus LAC cells protects against the killing activity of histones. A, to determine the MBC of histones, cells of S. aureus LAC were incubated with the indicated increasing amounts of individual histones. After incubation, cell mixtures were plated on BHI agar, and colony counts were determined. Cells of S. aureus LAC, the ΔfnbAfnbB mutant, the mutant overexpressing FnBPA or FnBPB, and clfA and the srtA mutants were tested in the MBC assay with histone H3 in B or histone H4 in C. After incubation, serial dilutions of the cell mixtures were plated onto BHI agar and colony counts determined.