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. 2019 Jan 8;294(10):3588–3602. doi: 10.1074/jbc.RA118.005707

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© 2019 Pietrocola et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 9. — Comparison of MBC values of H3 for S. pyogenes and S. aureus strains. A, to evaluate the potential attenuation of microbicidal activity of histone H3 by soluble FnBPB(163–480), cells of S. aureus ΔfnbAfnbB mutant were tested in the MBC assay for killing by H3 in the presence of recombinant FnBPB(163–480) or its variants. After incubation, serial dilutions of the cell mixtures were plated on BHI agar and colonies counted. The MBC for WT is reported as control. Data are the mean values ± S.D. from three independent experiments. Statistically significant differences compared with the WT are indicated (Student's t test; *, p < 0.05). B, to determine the neutralizing effect of soluble FnBPB(163–480) on the bactericidal effect of H3 for S. pyogenes 71-695 and S. aureus LAC, bacteria were incubated in the presence/absence of FnBPB(163–480). Following incubation, serial dilutions of the mixtures were plated on BHI agar and colonies counted. Data shown are the mean values ± S.D. from three independent experiments. Statistically significant differences compared with the untreated WT are indicated (Student's t test; *, p < 0.05). C, protective effect of FnBPB expression against cytotoxicity of histone H3 was assessed by evaluating the MBC against different S. aureus strains and their fnb mutants.