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. 2018 Nov 30;294(10):3406–3418. doi: 10.1074/jbc.RA118.005298

Figure 4.

Figure 4.

AmeloD has no effect on cell proliferation in IEE and in the CLDE dental epithelial cell line. A, immunostaining of Ki67 in P1 WT and AmeloD-KO incisors. Green, Ki67; blue, DAPI. Arrowheads indicate IEE cells. B, EdU staining of E18 WT and AmeloD-KO molars. C, quantification of EdU-positive cells. The EdU-positive ratio was calculated by counting the numbers of EdU-positive cells/stained nuclei in the epithelium. No significant difference was observed between WT and AmeloD-KO molars (n = 4). The mean is shown as lines. Error bars represent S.D. n.s., p > 0.05 with a two-tailed t test. D, AmeloD mRNA expression levels in AmeloD- and control mock vector–transfected CLDE cells (n = 3). Error bars represent S.D. **, p < 0.01 with a two-tailed t test. E, proliferation assay of AmeloD- and mock-transfected cells. The proliferation activity was detected as absorbance at 450 nm using a MMT kit (Dojindo; n = 3). Error bars represent S.D. n.s., p > 0.05 with two-way ANOVA. F, EdU incorporation and staining in AmeloD- and mock-transfected CLDE cells. Green, EdU; blue, Hoechst (n = 10). The mean is shown as lines. Error bars represent S.D. n.s., p > 0.05 with a two-tailed t test. Bars, 50 μm.