Figure 4.

Backbone amide dynamics and solvent-exchange of FABP1 in the absence and presence of GW7647. Comparison of ¹⁵N-¹H heteronuclear NOE values for apo and holo WT FABP1 (A) and K57A/E77A/K96A triple-mutant FABP1 (B) plotted against amino acid residue number. C, backbone amide resonance intensity ratio between apo and holo WT FABP1 plotted against the amino acid residue number. D, CLEANEX intensity values of apo and holo WT FABP1 plotted against amino acid residue number. Assignments that were missing or ambiguous due to peak overlap are highlighted with an asterisk.