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. 2019 Jan 3;294(10):3532–3548. doi: 10.1074/jbc.RA118.005423

Figure 4.

Figure 4.

RNA granules formed by S-scaffolds, but not R-scaffolds, with faster exchange rates dynamically change their shapes. A, methods for the quantification of granule shape change. Granules of interest (orange squares) were converted into binary images. Twenty one images (n = 0–20) taken over 100 s were merged, and the area of the merged granule was compared with the average area of the granule. Dotted lines, nuclei. Scale bar, 10 μm. B, granule shape change rates over 100 s. n = 7 cells. *, p < 0.05; **, p < 0.01; ***, p < 0.005, one-way ANOVA (F(7,48) = 5.99, p = 4.82e-5) followed by the Tukey-Kramer test. C, relationship between τ½ in the FRAP analysis and the granule shape change rates of the scaffolds. See also Movies S11 and S12.