Figure 7.
Mobile fraction of R-granule scaffolds is increased by being combined with S-granule scaffolds. A, representative time-lapse FRAP images of R- and S-scaffolds (FMR1 and RNG105) co-expressed in the same cell. Orange circles indicate the region bleached just before 0 s. Dotted line in the left panel, nucleus. Scale bar, 10 μm. B, representative FRAP curves fitted with exponential equations. C, halftime of recovery (τ½) and the mobile fraction (Fm) extracted from the fitted equations. + and − indicate with and without co-expression, respectively. n = 8. **, p < 0.01, Student's t test. D, τ½ and Fm of R-scaffolds (FMR1, Pumilio1, FUS, and TIA-1) with and without the co-expression of S-scaffolds (RNG105 and G3BP1). E, τ½ and Fm of S-scaffolds (RNG105 and G3BP1) with and without the co-expression of R-scaffolds (FMR1, Pumilio1, FUS, and TIA-1). D and E, top tables, the mean ± S.D. Bottom tables, statistical analysis. ↑, p < 0.05; ↑↑, p < 0.01; ↑↑↑, p < 0.005; ↑↑↑↑, p < 0.001, significant increase; ↓, p < 0.01, significant decrease; −, no significance, Student's t test with control. n = 8. F, model of the decrease in the immobile fraction of R-scaffolds by being combined with S-scaffolds.