Figure 10.

No pro-inflammatory response was detected when Met(O)-ApoA-I was provided in the form of reconstituted HDL. Unprimed BMDMs were incubated with apoA-I and apoA-I/rHDL preparations (1 μm) for 4 h. Pro-IL-1β levels were measured in cell extracts by Western blot analysis. A representative Western blot analysis with β-actin as a loading control is shown in the inset. Vertical black lines in the Western blots indicate positions where unnecessary lanes were removed from the original scans. Values are means and S.D. (error bars) of four independent determinations from two independent experiments. t test significance values are reported as p < 0.001 versus nonoxidized apoA-I (***).