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Methionine oxidation in apoA-I samples as measured by LC-MS
| Sample name | Oxidation system | H2O2/apoA-I | Percentage of oxidized methionine (mean ± S.D.) |
||
|---|---|---|---|---|---|
| Met(O)-86 | Met(O)-112 | Met(O)-148 | |||
| mol/mol | % | % | % | ||
| ApoA-I control | None | 0:1 | 11.2 ± 0.0 | 6.2 ± 1.1 | 1.6 ± 0.0 |
| Met(O)-ApoA-I | H2O2 | 200:1 | 57.2 ± 0.6 | 97.5 ± 0.1 | 29.7 ± 0.0 |
| Met(O)-ApoA-I | H2O2 | 500:1 | 94.3 ± 0.2 | 99.7 ± 0.1 | 85.7 ± 0.7 |
| Met(O)-ApoA-I | H2O2 | 1000:1 | 98.9 ± 0.2 | 99.5 ± 0.2 | 99.0 ± 0.1 |
