Figure 8.

Heterozygous down-regulation of YAP inhibits Akt phosphorylation after 1 week of TAC. WT and YAP-CHKO mice were subjected to either TAC or sham operation for 7 days. A, whole-heart lysates were subjected to immunoblotting for Thr-308–phosphorylated and total Akt or Ser-9–phosphorylated and total GSK-3β antibody. GAPDH antibody was used as loading control. Representative immunoblots are shown. B, quantification of relative pAkt/Akt shown in A. C, quantification of relative pGSK-3β/GSK-3β shown in A. B and C, data are normalized by the mean value in the WT-sham group. Values are mean ± S.D. n = 6 animals per group. *, p < 0.05; **, p < 0.01 versus sham; ##, p < 0.01; ###, p < 0.0005, compared with WT-TAC and YAP-CHKO-TAC. Statistical analyses were conducted with ANOVA. Post hoc analysis was conducted with Tukey's test.