Table 1.

Selection of samples for free thiol profile detection by DTNB addition (+197 ± 15 Da increase in mass) of the new mutations in the thiol variants

Shown are predicted molecular mass post-DTNB treatment for conjugation efficiency measurements using MS spectra. Difference shows actual measured mass minus theoretical mass. (For the complete sample set, see Table S2).

Variant description	Theoretical mass	Mass post-DTNB treatment
		Theoretical	Actual measured	Difference
	Da		Da
C34A/K93C	66,382	66,579	66,588	9
C34A/E294C	66,381	66,578	66,581	3