Table 2.
Selection of samples for GP-HPLC aggregation screening indicates monomeric stability over 6 months at 2–8 °C
Table shows percentage of monomers at timepoints 0 weeks, 7 weeks and 6 months. Parentheses shows percentage monomer compared to WT sample at the given timepoint. For the complete sample set, see Tables S3 and S4.
| Sample | GP-HPLC concentration | Monomer percentage |
Change in monomer percentage |
|||
|---|---|---|---|---|---|---|
| t = 0 | t = 7 weeks | t = 6 months | 0–7 weeks | 0–6 months | ||
| mg/ml | % | Δ% | ||||
| WT rHSA | 1.1 | 87 (100) | 88 (100) | 89 (100) | 1 | 2 |
| C34A/K93C | 0.7 | 91 (105) | 92 (105) | 92 (103) | 1 | 1 |
| C34A/E294C | 0.9 | 96 (110) | 96 (109) | 96 (108) | 0 | 0 |