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Single conjugation of maleimide-PEG2-biotin (+525 Da) during overnight incubation
Shown is a selection of samples for conjugation efficiency measured by the weight increase using mass spectrometry. (For the complete sample set, see Table S5.)
| Sample description | Reference molecular mass unconjugated | Theoretical conjugate mass | Conjugate intact mass | Conjugation |
|---|---|---|---|---|
| Da | Da | Da | % | |
| WT rHSA | 66,439 | 66,964 | 66,966 | 93 |
| C34A/K93C | 66,382 | 66,907 | 66,908 | 100 |
| C34A/E294C | 66,381 | 66,906 | 66,909 | >95 |
