Table 4.
Monomeric stability of combinations of cysteine mutations in the thiol variants during long-term storage at 5 °C by GP-HPLC, maintaining >95% monomer over 24 months
| Sample description | Percentage monomer |
||||||
|---|---|---|---|---|---|---|---|
| 0 months | 1 month | 2 months | 3 months | 6 months | 12 months | 24 months | |
| % | % | % | % | % | % | % | |
| WT rHSA | 100 | 99 | 100 | 100 | 100 | 99 | 100 |
| Double-thiol (C34 + K93C) | 100 | 99 | 99 | 99 | 98 | 97 | 97 |
| Double-thiol (C34 + E294C) | 100 | 99 | 99 | 99 | 99 | 97 | 98 |
| Triple-thiol (C34 + K93C + E294C) | 99 | 99 | 98 | 98 | 97 | 95 | 95 |
| Triple-thiol HBI variant (C34 + K93C + E294C + K573P) | 100 | 99 | 99 | 99 | 98 | 98 | 96 |