Table 5.

Labeling efficiency of AF680 dye post-conjugation, as measured by GP-HPLC dual-wavelength ratio determination (680 nm/280 nm)

Sample description	Monomer concentration	280-nm monomer peak area [mAU]	680-nm monomer peak area [mAU]	Ratio
	mg/ml
WT-1xAF680	1.989	3,162,386	4,222,436	1.34
WT-3xAF680	2.009	3,185,808	11,835,297	3.72
NB-3xAF680	1.991	3,117,158	13,434,647	4.31
HBII-3xAF680	2.029	3,238,326	11,848,510	3.66