Figure 4.

DSC curves of AGXA without Ca²⁺ (A) and with Ca²⁺ (B) were fitted using a non-two-state model. The T_m, ΔH and ΔH_v for AGXA without Ca²⁺ were 67.3 °C, 238.3 kcal.mol^-1 and 236.7 kcal.mol^-1, respectively. The T_m, ΔH and ΔH_v for AGXA with Ca²⁺ were 
77.8 °C, 290.7 kcal.mol^-1 and 282.6 kcal.mol^-1, respectively. Ca²⁺ ions caused a thermal increase (∼10 °C and ∼52 kcal.mol^-1) in T_m and ΔH, and the values of ΔH/ΔH_v were approximately equal to 1.0 for AGXA both with Ca²⁺ and without Ca²⁺ ions.