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. 2019 Feb;26(2):148–157. doi: 10.2174/0929866526666190116162958

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© 2019 Bentham Science Publishers

This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

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The CD-temperature profiles of AGXA without Ca²⁺ (A) and with Ca²⁺ (B) from 35 °C to 85 °C were calculated with Global 3 software. The spectra with absorbance above 2.0AU were excluded. The results showed that the unfolding temperatures (T_m) were 67.5 °C and 77.5 °C for AGXA without Ca²⁺ and with Ca²⁺, respectively. (The color version of the figure is available in the electronic copy of the article).