EphA4 registry with developing LCIC neurochemical framework at P8. LCIC digital merges (GAD = green, EphA4 = red, CR = blue) at low (a) and high magnification (b, inset box in a). Similar to P4, multiple GAD/EphA4-positive modules are readily apparent spanning LCIC layer 2 (b, arrowheads), with surrounding complementary CR extra modular labeling. Scale bar in (a) = 200 µm and (b) = 100 µm.