Figure 10.
CRY-1 and HIF-1α Show an Opposite Role on Cell Proliferation and Migration
(A) Representative HIF-1α western blots in WT MEFs, ΔCRY1, ΔHIF-1α, and ΔCRY1/ΔHIF-1α MEFs.
(B) Proliferation rate of WT, ΔCRY1, ΔHIF-1α, and ΔCRY1/ΔHIF-1α cells quantified by dynamic imaging using percentage of confluence at 3-h time intervals.
(C) Chart of 24- and 36-h time points. Statistics, Student's t test for paired values: *significant difference, p ≤ 0.01.
(D) Quantification of the colony formation ability of WT, ΔCRY1, ΔHIF-1α, and ΔCRY1/ΔHIF-1α cells as measured by soft agar assay. The cells were plated in soft agar and allowed to grow for 2 weeks under hypoxia. Cell growth was measured using Resazurin staining in a microplate reader with an excitation wavelength of 584 nm and emission at 612 nm. The OD612 in WT MEFs was set to 100%. Statistics, Student's t test for paired values: *significant difference, p < 0.05.
(E) Representative images of the wound recovery of WT, ΔCRY1, ΔHIF-1α, and ΔCRY1/ΔHIF-1α cells at 0, 12, and 24 h after introduction of the wound. Scale bar, 300 μm.
(F) Chart of 12- and 24-h time points. Statistics, Student's t test for paired values: *significant difference, p < 0.01.