Figure 2.
The Circadian Oscillation in NIH3T3 Cells Is Affected by HIF-1α
(A and B) (A) Representative examples of the bioluminescence oscillations in NIH3T3mPer2:Luc cells precultured under hypoxia (3% O2) for 32 h. (B) Graphical illustration of the amplitude (y axis) and period length (x axis) upon hypoxia treatment. Data are mean ± SEM. *p < 0.05.
(C) Representative example of the bioluminescence oscillations in NIH3T3 cells cotransfected with an mBmal1:luciferase reporter construct and either empty vector (black line) or HIF-1α P/P/N (green line).
(D) Graphical illustration of the amplitude (y axis) and period length (x axis) upon overexpression of HIF-1α P/P/N. Data are mean ± SEM. *p < 0.05, ***p < 0.001.