Figure 3.

The Circadian Oscillation of PAI-1 Expression Is Affected by Hypoxia

(A) Representative example of the bioluminescence oscillations in cells stably transfected with a PAI-1 promoter-driven luciferase construct under normoxia and hypoxia.

(B and C) (B) Graphical illustration of the amplitude (y axis) and period length (x axis) of PAI-1 promoter-driven luciferase expression under normoxia and hypoxia. Data are mean ± SEM. *p < 0.05. (C) Representative western blot analysis of total cell lysates of synchronized Rat-1 fibroblasts probed with PAI-1 and β-actin antibodies.

(D) Densitometric analysis of the PAI-1 protein levels presented on (C).