Fig. 6.

H₂O₂ accumulation and ROS-scavenging enzymatic activity in response to Cd stress. (A) H₂O₂ production observed via 3, 3′-diaminobenzidine staining in leaves of WT, CaWRKY41-OE1, and CaWRKY41-OE4 plants at 24 h post treatment with 50 µM CdSO₄. CK, control untreated. (B) Seedling H₂O₂ content. DPT, days post treatment. (C) Peroxidase (POD) activity. (D) Catalase (CAT) activity. (E) Ascorbate peroxidase (APX) activity. For B–E, 7-day-old WT, CaWRKY41-OE1, and CaWRKY41-OE4 seedlings were transferred to ½ MS medium without or with 25 µM CdSO₄ for 3 or 5 days before analysis. Data represent the mean ±SE of three biological replicates. Different letters indicate significant differences compared with the control (Tukey’s test; lowercase letters indicate P<0.05 and uppercase letters indicate P<0.01).