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. 2019 Mar 12;12:20. doi: 10.1186/s13041-019-0437-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Spatiotemporal expression of LAMP5 protein in various brain regions. a Spatial distribution of LAMP5 protein and Lamp5 mRNA at P56. Total protein lysates and total RNA of indicated regions were subjected to western blotting (upper panel) and RT-PCR analyses (lower panel), respectively. Midbrain was the remaining of midbrain after the superior and inferior colliculi were removed. Amido black staining and Gapdh are shown as loading controls. b and c Developmental expression of LAMP5. LAMP5 expression in whole brains at P0, P3, P7, P14, P22 and P56 (b), and in the striatum, cerebellum, pons and medulla oblongata at P7, P14, P22 and P56 was analyzed (c)