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. 2019 Mar 14;20:216. doi: 10.1186/s12864-019-5583-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Analysis of UMIs. a Stacked bar plot showing the fractions of unique on-target reads that are authentic (i.e., unique) and inflated (duplicated) due to UMI errors. Estimation was performed using UMI-tools. b Mean depth of unique coverage according to the length of the UMI sequence