FIG 5.

Collision-induced dissociation crotonobetainyl-CoA. (A) Extracted ion chromatograms correspond to the protonated form ([M+H]⁺) of crotonobetainyl-CoA at m/z 893.2066 (5-ppm accuracy). (B) Collision-induced dissociation tandem mass spectra (25% normalized collision energy). (1) Crotonobetainyl-CoA from the metabolome of GBB-grown cells. (2) Enzymatic formation of crotonobetainyl-CoA analyzed after 60 min in 100 μl of 50 mM Tris-HCl (pH 8.0) containing 3.9 μg of BcoC and 90 μM γ-butyrobetainyl-CoA 50, μM FAD, and 500 μM FC⁺PF⁻₆. (3) Crotonobetainyl-CoA reference standard. LC-MS analyses were conducted in the positive ionization mode.